Pseudomonas aeruginosaSmall Protease (PASP), a Keratitis Virulence Factor
نویسندگان
چکیده
منابع مشابه
Pseudomonas keratitis: protease IV gene conservation, distribution, and production relative to virulence and other Pseudomonas proteases.
PURPOSE To determine the distribution of the protease IV gene, the production of this and other proteases by multiple strains of Pseudomonas, and the virulence of a mutant specifically deficient in protease IV. METHODS The protease IV gene was cloned, its sequence analyzed, and its chromosomal location determined by pulse-field gel electrophoresis. Three PCR reactions were used to detect the ...
متن کاملPseudomonas keratitis. The role of an uncharacterized exoprotein, protease IV, in corneal virulence.
PURPOSE The role of exoproteins in the pathogenesis of Pseudomonas aeruginosa keratitis was investigated in three animal models by assessing the relationship between corneal virulence and the activities of exotoxin A, elastase, alkaline protease, and an uncharacterized protease, protease IV. METHODS The four Pseudomonal strains tested included a prototype strain (ATCC 27853) producing exotoxi...
متن کاملProperties of PASP: a Pseudomonas protease capable of mediating corneal erosions.
PURPOSE To analyze PASP in terms of its gene distribution and expression, its corneal pathologic effects, its enzymatic properties, and the protectiveness of the immune response to this protease. METHODS Twenty-five strains of P. aeruginosa were analyzed for the PASP gene and secreted protein by PCR and Western blot analysis, respectively. Active recombinant (r)PASP (10 microg/20 microL) or h...
متن کاملChelation in experimental Pseudomonas keratitis.
In cohtrast to its occasional occurrence during the first half of the 20th century (Joy, I942), Pseudomonas aeruginosa to-day ranks as a common, if not the most common, cause of bacterial corneal ulcer in the United States of America. Even with prompt laboratory diagnosis and use of adequate and specific antimicrobial therapy, the rapidly destructive lesion caused by this bacterium frequently r...
متن کاملSerrated Margins in Pseudomonas aeruginosa Keratitis
PURPOSE To describe 3 cases of culture-positive P. aeruginosa keratitis, who presented with a focus having serrated margins. METHODS Observational case report. RESULTS Three cases wearing contact lenses complained of symptoms such as pain, redness, and a decrease in vision. Slit-lamp biomicroscopic examination revealed a focus with serrated margins in all cases. The patients underwent corne...
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ژورنال
عنوان ژورنال: Investigative Opthalmology & Visual Science
سال: 2013
ISSN: 1552-5783
DOI: 10.1167/iovs.13-11788